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Crystal structure of a monomeric cyan fluorescent protein in the fluorescent state

X-ray diffraction
1.79Å resolution
PDB entry 2otb coloured by chain, front view.
PDB entry 2otb coloured by chain, side view.
PDB entry 2otb coloured by chain, top view.
Source organism: Clavularia sp.
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: GFP-like fluorescent chromoprotein cFP484
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Multimeric state: monomeric
Accessible surface area: 9433.3 Ã…2
Buried surface area: 636.34 Ã…2
Dissociation area: 318.17 Ã…2
Dissociation energy (ΔGdiss): -2.09 kcal/mol
Dissociation entropy (TΔSdiss): 3.96 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-193615
Assembly 2
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Assembly Name: GFP-like fluorescent chromoprotein cFP484
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Multimeric state: monomeric
Accessible surface area: 9411.97 Ã…2
Buried surface area: 634.88 Ã…2
Dissociation area: 317.44 Ã…2
Dissociation energy (ΔGdiss): -1.45 kcal/mol
Dissociation entropy (TΔSdiss): 3.94 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-193615

Macromolecules

GFP-like fluorescent chromoprotein cFP484
Chains: A, B
Length: 214 amino acids
Theoretical weight: 24.8 KDa
Source organism: Clavularia sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y3 (Residues: 44-259; Coverage: 81%)
Pfam: Green fluorescent protein
InterPro:
CATH: Green fluorescent protein
GFP-like fluorescent chromoprotein cFP484 in PDB entry 2otb, assembly 1, front view.
GFP-like fluorescent chromoprotein cFP484 in PDB entry 2otb, assembly 1, side view.
GFP-like fluorescent chromoprotein cFP484 in PDB entry 2otb, assembly 1, top view.
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