› 2otb

X-ray diffraction
1.79Å resolution

Crystal structure of a monomeric cyan fluorescent protein in the fluorescent state

Released:
DOI:
PDB entry 2otb coloured by chain, front view.
PDB entry 2otb coloured by chain, side view.
PDB entry 2otb coloured by chain, top view.
Source organism: Clavularia sp.
Primary publication:
Structural basis for reversible photobleaching of a green fluorescent protein homologue.
Henderson JN, Ai HW, Campbell RE, Remington SJ
Proc Natl Acad Sci U S A 104 6672-7 (2007)
PMID: 17420458

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-like fluorescent chromoprotein cFP484 Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 24.8 KDa
Source organism: Clavularia sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y3 (Residues: 44-259; Coverage: 81%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PIA 2 x PIA

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 65.016Å b: 67.736Å c: 95.382Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.252
Expression system: Escherichia coli

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Citations

34 review citations

Chudakov et al. (2010)
33 more

6 mentions without citation

Henderson et al. (2007)
5 more