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X-RAY CRYSTAL STRUCTURE OF THE CsPYL1-iSB9-HAB1 TERNARY COMPLEX

X-ray diffraction
1.9Å resolution
PDB entry 8ay3 coloured by chain, front view.
PDB entry 8ay3 coloured by chain, side view.
PDB entry 8ay3 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Protein phosphatase 2C 16 and Abscisic acid receptor PYL1
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Multimeric state: hetero dimer
Accessible surface area: 21530.91 Ã…2
Buried surface area: 3280.99 Ã…2
Dissociation area: 863.29 Ã…2
Dissociation energy (ΔGdiss): 0.51 kcal/mol
Dissociation entropy (TΔSdiss): 12.46 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-100358

Macromolecules

Abscisic acid receptor PYL1
Chain: A
Length: 209 amino acids
Theoretical weight: 23.29 KDa
Source organism: Citrus sinensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A067E666 (Residues: 1-209; Coverage: 100%)
Gene name: CISIN_1g046151mg
Pfam: Polyketide cyclase / dehydrase and lipid transport
InterPro:
Abscisic acid receptor PYL1 in PDB entry 8ay3, assembly 1, front view.
Abscisic acid receptor PYL1 in PDB entry 8ay3, assembly 1, side view.
Abscisic acid receptor PYL1 in PDB entry 8ay3, assembly 1, top view.
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Protein phosphatase 2C 16
Chain: B
Length: 333 amino acids
Theoretical weight: 36.97 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9CAJ0 (Residues: 179-511; Coverage: 68%)
Gene names: At1g72770, F28P22.4, HAB1, P2C-HA
Pfam: Protein phosphatase 2C
InterPro:
Protein phosphatase 2C 16 in PDB entry 8ay3, assembly 1, front view.
Protein phosphatase 2C 16 in PDB entry 8ay3, assembly 1, side view.
Protein phosphatase 2C 16 in PDB entry 8ay3, assembly 1, top view.
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