› 7apu

X-ray diffraction
1.36Å resolution

Structure of Adenylate kinase from Escherichia coli in complex with two ADP molecules refined at 1.36 A resolution.

Released:
DOI:
PDB entry 7apu coloured by chain, front view.
PDB entry 7apu coloured by chain, side view.
PDB entry 7apu coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Dynamic Connection between Enzymatic Catalysis and Collective Protein Motions.
Ojeda-May P, Mushtaq AU, Rogne P, Verma A, Ovchinnikov V, ³Ò°ù³Ü²Ô»å²õ³Ù°ùö³¾ C, Dulko-Smith B, Sauer UH, Wolf-Watz M, Nam K
OpenAccess logo Biochemistry 60 2246-2258 (2021)
PMID: 34250801

Function and Biology Details

Reaction catalysed: 
ATP + AMP = 2 ADP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 23.62 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P69441 (Residues: 1-214; Coverage: 100%)
Gene names: JW0463, adk, b0474, dnaW, plsA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 4 x ADP
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 72.754Å b: 82.226Å c: 78.783Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.206
Expression system: Escherichia coli K-12

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Citations

6 citation in other articles

Lu et al. (2022)
5 more

2 mentions without citation

Ojeda-May et al. (2021)
1 more