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The X-ray crystal structure of lymphocyte perforin

X-ray diffraction
2.75Å resolution
PDB entry 3nsj coloured by chain, front view.
PDB entry 3nsj coloured by chain, side view.
PDB entry 3nsj coloured by chain, top view.
Source organism: Mus musculus
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Perforin-1
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Multimeric state: monomeric
Accessible surface area: 25524.17 Ã…2
Buried surface area: 1959.81 Ã…2
Dissociation area: 76.88 Ã…2
Dissociation energy (ΔGdiss): -1.22 kcal/mol
Dissociation entropy (TΔSdiss): 1.46 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-145462

Macromolecules

Perforin-1
Chain: A
Length: 540 amino acids
Theoretical weight: 60.8 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10820 (Residues: 21-554; Coverage: 100%)
Gene names: Pfp, Prf1
Pfam:
InterPro:
CATH: C2 domain
Perforin-1 in PDB entry 3nsj, assembly 1, front view.
Perforin-1 in PDB entry 3nsj, assembly 1, side view.
Perforin-1 in PDB entry 3nsj, assembly 1, top view.
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