7wlt › Citations

Structure deformation and curvature sensing of PIEZO1 in lipid membranes.

Yang X, Lin C, Chen X, Li S, Li X, Xiao B
Nature 604 377-383 (2022)
Cited: 42 times
EuropePMC logo PMID:
doi logo

Abstract

PIEZO channels respond to piconewton-scale forces to mediate critical physiological and pathophysiological processes1-5. Detergent-solubilized PIEZO channels form bowl-shaped trimers comprising a central ion-conducting pore with an extracellular cap and three curved and non-planar blades with intracellular beams6-10, which may undergo force-induced deformation within lipid membranes11. However, the structures and mechanisms underlying the gating dynamics of PIEZO channels in lipid membranes remain unresolved. Here we determine the curved and flattened structures of PIEZO1 reconstituted in liposome vesicles, directly visualizing the substantial deformability of the PIEZO1-lipid bilayer system and an in-plane areal expansion of approximately 300 nm2 in the flattened structure. The curved structure of PIEZO1 resembles the structure determined from detergent micelles, but has numerous bound phospholipids. By contrast, the flattened structure exhibits membrane tension-induced flattening of the blade, bending of the beam and detaching and rotating of the cap, which could collectively lead to gating of the ion-conducting pathway. On the basis of the measured in-plane membrane area expansion and stiffness constant of PIEZO1 (ref. 11), we calculate a half maximal activation tension of about 1.9 pN nm-1, matching experimentally measured values. Thus, our studies provide a fundamental understanding of how the notable deformability and structural rearrangement of PIEZO1 achieve exquisite mechanosensitivity and unique curvature-based gating in lipid membranes.

Articles - 7wlt mentioned but not cited (2)

  1. Jiang W, Lacroix J, Luo YL. Biophys Rep (N Y) 2 100080 (2022)
  2. Peralta FA, Balcon M, Martz A, Biljali D, Cevoli F, Arnould B, Taly A, Chataigneau T, Grutter T. Nat Commun 14 1269 (2023)


Reviews citing this publication (15)

  1. Zheng Q, Liu H, Yu W, Dong Y, Zhou L, Deng W, Hua F. Brain Behav 13 e3136 (2023)
  2. Zhu Y, Zhang M, Sun Q, Wang X, Li X, Li Q. Polymers (Basel) 15 3255 (2023)
  3. Kong L, Gao X, Qian Y, Sun W, You Z, Fan C. Theranostics 12 4993-5014 (2022)
  4. Xin Y, Li K, Huang M, Liang C, Siemann D, Wu L, Tan Y, Tang X. Oncogene 42 3457-3490 (2023)
  5. Gobet A, Moissonnier L, Chaptal V. Methods Mol Biol 2715 471-483 (2024)
  6. Zhang X, Zhang Y, Su Q, Liu Y, Li Z, Yong VW, Xue M. Neurosci Bull (2023)
  7. Zhou Z, Martinac B. Int J Mol Sci 24 14113 (2023)
  8. Xiao R, Liu J, Xu XZS. Curr Opin Cell Biol 84 102216 (2023)
  9. Li X, Hu J, Yin P, Liu L, Chen Y. Heliyon 9 e19427 (2023)
  10. Xie P, Zhang H, Qin Y, Xiong H, Shi C, Zhou Z. Biomolecules 13 1772 (2023)
  11. Xu H, He Y, Hong T, Bi C, Li J, Xia M. Front Cardiovasc Med 9 1021540 (2022)
  12. Puljko B, Stojanović M, Ilic K, Kalanj-Bognar S, Mlinac-Jerkovic K. Biomedicines 10 1518 (2022)
  13. Yuan W, Zhang X, Fan X. Curr Issues Mol Biol 45 5830-5848 (2023)
  14. Bernareggi A, Bosutti A, Massaria G, Giniatullin R, Malm T, Sciancalepore M, Lorenzon P. Int J Mol Sci 23 6616 (2022)
  15. Davis MJ, Earley S, Li YS, Chien S. Physiol Rev 103 1247-1421 (2023)

Articles citing this publication (25)

  1. Haselwandter CA, Guo YR, Fu Z, MacKinnon R. Proc Natl Acad Sci U S A 119 e2208034119 (2022)
  2. Bryniarska-Kubiak N, Kubiak A, Basta-Kaim A. Curr Neuropharmacol 21 2030-2035 (2023)
  3. Sun Q, Pei F, Zhang M, Zhang B, Jin Y, Zhao Z, Wei Q. Adv Sci (Weinh) 10 e2204479 (2023)
  4. Yang S, Miao X, Arnold S, Li B, Ly AT, Wang H, Wang M, Guo X, Pathak MM, Zhao W, Cox CD, Shi Z. Nat Commun 13 7467 (2022)
  5. Pöhnl M, Trollmann MFW, Böckmann RA. Nat Commun 14 8038 (2023)
  6. Jiang W, Wijerathne TD, Zhang H, Lin YC, Jo S, Im W, Lacroix JJ, Luo YL. Proc Natl Acad Sci U S A 120 e2310933120 (2023)
  7. Nakayama Y, Rohde PR, Martinac B. Microorganisms 11 194 (2023)
  8. De Giorgio E, Giannios P, Espinàs ML, Llimargas M. PLoS Biol 21 e3001978 (2023)
  9. Weikl TR. Proc Natl Acad Sci U S A 119 e2214536119 (2022)
  10. Chang SS, Dijkman PM, Wiessing SA, Kudryashev M. Sci Rep 13 11523 (2023)
  11. Mulhall EM, Gharpure A, Lee RM, Dubin AE, Aaron JS, Marshall KL, Spencer KR, Reiche MA, Henderson SC, Chew TL, Patapoutian A. Nature 620 1117-1125 (2023)
  12. Ozkan AD, Wijerathne TD, Gettas T, Lacroix JJ. Cell Rep 42 112837 (2023)
  13. Li J, Fu Y, Zhang K, Li Y. Vaccines (Basel) 10 1562 (2022)
  14. Romero LO, Caires R, Kaitlyn Victor A, Ramirez J, Sierra-Valdez FJ, Walsh P, Truong V, Lee J, Mayor U, Reiter LT, Vásquez V, Cordero-Morales JF. Nat Commun 14 1167 (2023)
  15. Goodman MB, Haswell ES, Vásquez V. J Gen Physiol 155 e202213248 (2023)
  16. Tao X, Zhao C, MacKinnon R. Proc Natl Acad Sci U S A 120 e2302325120 (2023)
  17. Wijerathne TD, Ozkan AD, Lacroix JJ. J Gen Physiol 155 e202213260 (2023)
  18. Zhang J, Maksaev G, Yuan P. Nat Commun 14 6284 (2023)
  19. Qu J, Zong HF, Shan Y, Zhang SC, Guan WP, Yang Y, Zhao HL. Neural Regen Res 18 1750-1756 (2023)
  20. Andolfo I, Monaco V, Cozzolino F, Rosato BE, Marra R, Cerbone V, Pinto VM, Forni GL, Unal S, Iolascon A, Monti M, Russo R. Blood Adv 7 2681-2693 (2023)
  21. Comment Boucard AA. Nature 604 628-630 (2022)
  22. Mount J, Maksaev G, Summers BT, Fitzpatrick JAJ, Yuan P. Nat Commun 13 6904 (2022)
  23. Lee JH, Perez-Flores MC, Park S, Kim HJ, Chen Y, Kang M, Kersigo J, Choi J, Thai PN, Woltz RL, Perez-Flores DC, Perkins G, Sihn CR, Trinh P, Zhang XD, Sirish P, Dong Y, Feng WW, Pessah IN, Dixon RE, Sokolowski B, Fritzsch B, Chiamvimonvat N, Yamoah EN. Nat Commun 15 526 (2024)
  24. Bongrand P. Int J Mol Sci 24 2266 (2023)
  25. Wijerathne TD, Ozkan AD, Lacroix JJ. Proc Natl Acad Sci U S A 119 e2202269119 (2022)


Quick links