› 7eiy

X-ray diffraction
2.2Å resolution

Human histidine decarboxylase mutant Y334F soaking with histidine

Released:
DOI:
PDB entry 7eiy coloured by chain, front view.
PDB entry 7eiy coloured by chain, side view.
PDB entry 7eiy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of the HDC Y334F mutant
Komori H, Nitta Y
J Biol Macromol 21 69-74 (2021)

Function and Biology Details

Reaction catalysed: 
L-histidine = histamine + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidine decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 481 amino acids
Theoretical weight: 54.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19113 (Residues: 2-477; Coverage: 72%)
Gene name: HDC
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand HIS 2 x HIS
Ligand SO4 1 x SO4
1 modified residue:
Ligand CSX 2 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 57.93Å b: 84.998Å c: 187.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.166 0.224
Expression system: Escherichia coli

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