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ABA-mimicking ligand AMC1beta in complex with ABA receptor PYL2 and PP2C HAB1

X-ray diffraction
2.624Å resolution
PDB entry 5vt7 coloured by chain, front view.
PDB entry 5vt7 coloured by chain, side view.
PDB entry 5vt7 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Assemblies composition:
monomeric
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1
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Assembly Name: Abscisic acid receptor PYL2
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Multimeric state: monomeric
Accessible surface area: 9322.99 Ã…2
Buried surface area: 0.0 Ã…2
Dissociation area: 0 Ã…2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-131332
Assembly 2
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Assembly Name: Protein phosphatase 2C 16
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Multimeric state: monomeric
Accessible surface area: 14121.42 Ã…2
Buried surface area: 210.85 Ã…2
Dissociation area: 7.26 Ã…2
Dissociation energy (ΔGdiss): 2.92 kcal/mol
Dissociation entropy (TΔSdiss): -1.47 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-219661
Assembly 3 (preferred)
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Assembly Name: Protein phosphatase 2C 16 and Abscisic acid receptor PYL2
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Multimeric state: hetero dimer
Accessible surface area: 21814.57 Ã…2
Buried surface area: 1840.69 Ã…2
Dissociation area: 814.92 Ã…2
Dissociation energy (ΔGdiss): 1.06 kcal/mol
Dissociation entropy (TΔSdiss): 12.4 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-131333

Macromolecules

Abscisic acid receptor PYL2
Chain: A
Length: 177 amino acids
Theoretical weight: 19.91 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O80992 (Residues: 14-188; Coverage: 92%)
Gene names: At2g26040, PYL2, RCAR14, T19L18.15
Pfam: Polyketide cyclase / dehydrase and lipid transport
InterPro:
CATH: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4
Abscisic acid receptor PYL2 in PDB entry 5vt7, assembly 3, front view.
Abscisic acid receptor PYL2 in PDB entry 5vt7, assembly 3, side view.
Abscisic acid receptor PYL2 in PDB entry 5vt7, assembly 3, top view.
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Protein phosphatase 2C 16
Chain: B
Length: 341 amino acids
Theoretical weight: 37.83 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9CAJ0 (Residues: 172-511; Coverage: 70%)
Gene names: At1g72770, F28P22.4, HAB1, P2C-HA
Pfam: Protein phosphatase 2C
InterPro:
CATH: PPM-type phosphatase domain
Protein phosphatase 2C 16 in PDB entry 5vt7, assembly 3, front view.
Protein phosphatase 2C 16 in PDB entry 5vt7, assembly 3, side view.
Protein phosphatase 2C 16 in PDB entry 5vt7, assembly 3, top view.
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