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ABA-mimicking ligand N-(1-METHYL-2-OXO-1,2,3,4-TETRAHYDROQUINOLIN-6-YL)-1-(4-METHYLPHENYL)METHANESULFONAMIDE in complex with ABA receptor PYL2 and PP2C HAB1

X-ray diffraction
3.15Å resolution
PDB entry 4lgb coloured by chain, front view.
PDB entry 4lgb coloured by chain, side view.
PDB entry 4lgb coloured by chain, top view.
Source organism: Arabidopsis thaliana
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Protein phosphatase 2C 16 and Abscisic acid receptor PYL2
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Multimeric state: hetero dimer
Accessible surface area: 21659.41 Ã…2
Buried surface area: 2539.7 Ã…2
Dissociation area: 945.94 Ã…2
Dissociation energy (ΔGdiss): 14.77 kcal/mol
Dissociation entropy (TΔSdiss): 12.41 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-131333

Macromolecules

Abscisic acid receptor PYL2
Chain: A
Length: 177 amino acids
Theoretical weight: 19.91 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O80992 (Residues: 14-188; Coverage: 92%)
Gene names: At2g26040, PYL2, RCAR14, T19L18.15
Pfam: Polyketide cyclase / dehydrase and lipid transport
InterPro:
CATH: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4
Abscisic acid receptor PYL2 in PDB entry 4lgb, assembly 1, front view.
Abscisic acid receptor PYL2 in PDB entry 4lgb, assembly 1, side view.
Abscisic acid receptor PYL2 in PDB entry 4lgb, assembly 1, top view.
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Protein phosphatase 2C 16
Chain: B
Length: 341 amino acids
Theoretical weight: 37.83 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CAJ0 (Residues: 172-511; Coverage: 70%)
Gene names: At1g72770, F28P22.4, HAB1, P2C-HA
Pfam: Protein phosphatase 2C
InterPro:
CATH: PPM-type phosphatase domain
Protein phosphatase 2C 16 in PDB entry 4lgb, assembly 1, front view.
Protein phosphatase 2C 16 in PDB entry 4lgb, assembly 1, side view.
Protein phosphatase 2C 16 in PDB entry 4lgb, assembly 1, top view.
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