› 3wg8

X-ray diffraction
2.3Å resolution

Crystal structure of the abscisic acid receptor PYR1 in complex with an antagonist AS6

Released:
DOI:
PDB entry 3wg8 coloured by chain, front view.
PDB entry 3wg8 coloured by chain, side view.
PDB entry 3wg8 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Designed abscisic acid analogs as antagonists of PYL-PP2C receptor interactions.
Takeuchi J, Okamoto M, Akiyama T, Muto T, Yajima S, Sue M, Seo M, Kanno Y, Kamo T, Endo A, Nambara E, Hirai N, Ohnishi T, Cutler SR, Todoroki Y
Nat Chem Biol 10 477-82 (2014)
PMID: 24792952

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-129274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Abscisic acid receptor PYR1 Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25.43 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O49686 (Residues: 1-191; Coverage: 100%)
Gene names: ABIP6, At4g17870, PYR1, RCAR11, T6K21.50
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

1 bound ligand:
Ligand 6AS 1 x 6AS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P3121
Unit cell:
a: 66.932Å b: 66.932Å c: 78.237Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.238 0.281
Expression system: Escherichia coli

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Citations

10 review citations

Lievens et al. (2017)
9 more

3 mentions without citation

Zhang et al. (2015)
2 more