Structure analysis

Complex structure of (+)-ABA-bound PYL1 and ABI1

X-ray diffraction
1.878Å resolution
Source organism: Arabidopsis thaliana
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero dimer
Accessible surface area: 20036.97 Ã…2
Buried surface area: 1822.26 Ã…2
Dissociation area: 846.02 Ã…2
Dissociation energy (ΔGdiss): 1.87 kcal/mol
Dissociation entropy (TΔSdiss): 12.34 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-155965

Macromolecules

Chain: A
Length: 202 amino acids
Theoretical weight: 23.33 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8VZS8 (Residues: 20-221; Coverage: 91%)
Gene names: At5g46790, MZA15.21, PYL1, RCAR12
Pfam: Polyketide cyclase / dehydrase and lipid transport
InterPro:
CATH: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

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Chain: B
Length: 316 amino acids
Theoretical weight: 34.79 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49597 (Residues: 119-434; Coverage: 73%)
Gene names: ABI1, At4g26080, F20B18.190
Pfam: Protein phosphatase 2C
InterPro:
CATH: PPM-type phosphatase domain

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