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Crystallographic refinement of ricin to 2.5 Angstroms

X-ray diffraction
2.5Å resolution
PDB entry 2aai coloured by chain, front view.
PDB entry 2aai coloured by chain, side view.
PDB entry 2aai coloured by chain, top view.
Source organism: Ricinus communis
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Ricin A chain
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Multimeric state: hetero dimer
Accessible surface area: 22618.97 Ã…2
Buried surface area: 6397.4 Ã…2
Dissociation area: 1,667.48 Ã…2
Dissociation energy (ΔGdiss): 5.38 kcal/mol
Dissociation entropy (TΔSdiss): 13.17 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-136288

Macromolecules

Ricin A chain
Chain: A
Length: 267 amino acids
Theoretical weight: 29.94 KDa
Source organism: Ricinus communis
Expression system: Not provided
UniProt:
  • Canonical: P02879 (Residues: 36-302; Coverage: 49%)
Pfam: Ribosome inactivating protein
InterPro:
CATH:
SCOP: Plant cytotoxins
Ricin A chain in PDB entry 2aai, assembly 1, front view.
Ricin A chain in PDB entry 2aai, assembly 1, side view.
Ricin A chain in PDB entry 2aai, assembly 1, top view.
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Ricin B chain
Chain: B
Length: 262 amino acids
Theoretical weight: 28.99 KDa
Source organism: Ricinus communis
Expression system: Not provided
UniProt:
  • Canonical: P02879 (Residues: 315-576; Coverage: 48%)
Pfam: Ricin-type beta-trefoil lectin domain
InterPro:
CATH: Trefoil (Acidic Fibroblast Growth Factor, subunit A)
SCOP: Ricin B-like
Ricin B chain in PDB entry 2aai, assembly 1, front view.
Ricin B chain in PDB entry 2aai, assembly 1, side view.
Ricin B chain in PDB entry 2aai, assembly 1, top view.
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