Project PXD054720
Summary
ABRF iPRG 2023 crosslinking study - Mascot COMPLETE results
The three proteomics research groups, Proteomics (PRG), Proteome Informatics (iPRG) and Proteomics Standards (sPRG), have all held interesting studies over the years. This year (2023) the iPRG group has put together a crosslinking study. The goal is to teach people how to analyze crosslinked samples with existing software and provide them with data sets that they can practice on. It is then hoped ...
Read moreThe study consists of recombinant proteins that were purified from an E. coli expression system. The expressed proteins form complexes under optimal conditions. The preparations were crosslinked with Disuccinimidyl sulfoxide (DSSO), ultracentrifuged and separated by SDS-PAGE. Bands were excised before reduction and alkylation with iodoacetamide and digestion with trypsin. The digests were analyze...
Read moreThree data files have been used for tutorials on different software analysis tools as phase 1 of the study. Matrix Science has been fortunate to be invited to provide a tutorial (https://www.matrixscience.com/downloads/ABRF2023/ABRF_iPRG_2022%20study_XLink%20tutorial_Mascot.pdf) for the analysis using Mascot Server and Distiller. The data is high resolution MS/MS data and we processed them with M...
Read moreDr Yasset Perez-Riverol, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Cambridge, UK (lab head)
08/08/2024
08/08/2024
Publication
Publication pending
Visualizations
Name | Type | View |
|---|
F001234-mzid-gz | cross-linking | |
F001235-mzid-gz | cross-linking | |
F001234-mzid | cross-linking | |
F001235-mzid | cross-linking | |
F001236-mzid | cross-linking | |
F001236-mzid-gz | cross-linking |
Project Files
Name | Type | Size (M) | Download | # |
|---|
F001236.mgf | PEAK | 63 | c00eb3f64b8cd45ec4e87a759e19c47d06dbc82de781ba741e558bbbb296575e | |
F001235.mzid.gz | RESULT | 1 | 28cafb5ae54e21bf713868cd201082203886f1fc5f1c146aaa8d161da723d6aa | |
checksum.txt | OTHER | 1227 bit | 0befbfde0c6f6100af5df3555f84a33c96cf43255ef6fdfc6eb71b8511da7b26 | |
211026EWas02_F1.raw | RAW | 506 | 7aa1211e2c91ba350063c6b2040aa39bb035e9fd7ed76eb09dca29b965455c6c | |
iPRG_2023_Crosslinking_Study_Letter.docx | OTHER | 21685 bit | 43abef67568e23b3c19e83700c188250832084af3a00def44c5bddc4d4aa77c5 | |
F001234.mzid.gz | RESULT | 1 | 15b982cca6a8762d60510128e1d2ff012ec4529754fbf5edfe9b3e1c2c1c5aba | |
211026EWas03_F2.raw | RAW | 509 | 57f8e6a8de34c1ebcdd74c55daaa697d5624e9b14eec0895196a4382026c0acd | |
211026EWas01_E1.raw | RAW | 488 | c6edb4a00a3e650809b52883a4d35ca950c9f2f73d3f01e99175b8434324d831 | |
ABRF_iPRG_XL_2023.fasta | OTHER | 2 | 9e9cec36964575c67a7bab96e2d8f6ea603d016f0652fcb83b05aaaf4c5fcaa1 | |
F001236.mzid.gz | RESULT | 1 | cdaf9bc8d3d24704b8419020947072fff60cbeaa8bf60fabdd169f8bfe761414 | |
F001234.mgf | PEAK | 60 | 0b1d79b6ed0790b4a158b01889dde4d64e6f4d9f800cee6c1b65257cab2e830d | |
F001235.mgf | PEAK | 69 | 00426abe5dcec6fb752f265716463756d3a33ae381369a1e280b47aa67f57d13 |
- Total 12 items
- 1
Properties
Unknown
Unknown
Unknown
Chemical cross-linking coupled with mass spectrometry proteomics
Bottom-up proteomics
Data-dependent acquisition
Unknown
Not available
RAW (3), SEARCH (0), PEAK (3), RESULT (3), OTHER (3)
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