› 6fhv

X-ray diffraction
2Å resolution

Crystal structure of Penicillium oxalicum Glucoamylase

Released:
DOI:
PDB entry 6fhv coloured by chain, front view.
PDB entry 6fhv coloured by chain, side view.
PDB entry 6fhv coloured by chain, top view.
Source organism: Penicillium oxalicum 114-2
Primary publication:
Structural insight into industrially relevant glucoamylases: flexible positions of starch-binding domains.
Roth C, Moroz OV, Ariza A, Skov LK, Ayabe K, Davies GJ, Wilson KS
Acta Crystallogr D Struct Biol 74 463-470 (2018)
PMID: 29717717

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glucoamylase Chain: A
Molecule details ›
Chain: A
Length: 594 amino acids
Theoretical weight: 65.45 KDa
Source organism: Penicillium oxalicum 114-2
Expression system: Penicillium oxalicum
UniProt:
  • Canonical: S7ZIW0 (Residues: 23-615; Coverage: 100%)
Gene name: PDE_05527
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
4 bound ligands:
Ligand B3P 1 x B3P
Ligand PG4 1 x PG4
Ligand PEG 1 x PEG
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: R32
Unit cell:
a: 189.253Å b: 189.253Å c: 115.379Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.187 0.219
Expression system: Penicillium oxalicum

Quick links

Citations

2 review citations

Sidar et al. (2020)
1 more

2 mentions without citation

Tong et al. (2021)
1 more