› 4u7o

X-ray diffraction
2.4Å resolution

Active histidine kinase bound with ATP

Released:
DOI:
PDB entry 4u7o coloured by chain, front view.
PDB entry 4u7o coloured by chain, side view.
PDB entry 4u7o coloured by chain, top view.
Source organism: Lactiplantibacillus plantarum JDM1
Primary publication:
Conformational dynamics of the essential sensor histidine kinase WalK.
Cai Y, Su M, Ahmad A, Hu X, Sang J, Kong L, Chen X, Wang C, Shuai J, Han A
OpenAccess logo Acta Crystallogr D Struct Biol 73 793-803 (2017)
PMID: 28994408

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 277 amino acids
Theoretical weight: 31.65 KDa
Source organism: Lactiplantibacillus plantarum JDM1
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M3KKX3 (Residues: 1-193; Coverage: 100%)
Gene names: JDM1_0052, hpk1
Sequence domains: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AN2 2 x AN2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 54.528Å b: 98.389Å c: 116.369Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.239
Expression system: Escherichia coli

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Citations

3 review citations

Jacob-Dubuisson et al. (2018)
2 more

6 mentions without citation

Gourinchas et al. (2019)
5 more