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Functional mechanism of the abscisic acid agonist pyrabactin.

Hao Q, Yin P, Yan C, Yuan X, Li W, Zhang Z, Liu L, Wang J, Yan N
J Biol Chem 285 28946-52 (2010)
Cited: 25 times
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Abstract

Pyrabactin is a synthetic abscisic acid (ABA) agonist that selectively inhibits seed germination. The use of pyrabactin was pivotal in the identification of the PYR1/PYL/RCAR family (PYL) of proteins as the ABA receptor. Although they both act through PYL proteins, pyrabactin and ABA share no apparent chemical or structural similarity. It remains unclear how pyrabactin functions as an ABA agonist. Here, we report the crystal structure of pyrabactin in complex with PYL1 at 2.4 A resolution. Structural and biochemical analyses revealed that recognition of pyrabactin by the pocket residues precedes the closure of switch loop CL2. Structural comparison between pyrabactin- and ABA-bound PYL1 reveals a general principle in the arrangements of function groups of the two distinct ligands. The study provides a framework for the development of novel ABA agonists that may have applicable potentials in agriculture.

Reviews - 3neg mentioned but not cited (1)

  1. Hewage KAH, Yang JF, Wang D, Hao GF, Yang GF, Zhu JK. Adv Sci (Weinh) 7 2001265 (2020)

Articles - 3neg mentioned but not cited (1)

  1. Hao Q, Yin P, Yan C, Yuan X, Li W, Zhang Z, Liu L, Wang J, Yan N. J Biol Chem 285 28946-28952 (2010)


Reviews citing this publication (8)

  1. Weiner JJ, Peterson FC, Volkman BF, Cutler SR. Curr Opin Plant Biol 13 495-502 (2010)
  2. Miyakawa T, Fujita Y, Yamaguchi-Shinozaki K, Tanokura M. Trends Plant Sci 18 259-266 (2013)
  3. Ng LM, Melcher K, Teh BT, Xu HE. Acta Pharmacol Sin 35 567-584 (2014)
  4. Guo J, Yang X, Weston DJ, Chen JG. J Integr Plant Biol 53 469-479 (2011)
  5. Melcher K, Zhou XE, Xu HE. Curr Opin Struct Biol 20 722-729 (2010)
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  7. Balcerowicz M, Shetty KN, Jones AM. Plant Physiol 187 590-602 (2021)
  8. Miyakawa T, Tanokura M. Biophysics (Nagoya-shi) 7 123-128 (2011)

Articles citing this publication (15)

  1. Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, Frommer WB. Elife 3 e01741 (2014)
  2. Ng LM, Soon FF, Zhou XE, West GM, Kovach A, Suino-Powell KM, Chalmers MJ, Li J, Yong EL, Zhu JK, Griffin PR, Melcher K, Xu HE. Proc Natl Acad Sci U S A 108 21259-21264 (2011)
  3. Takeuchi J, Okamoto M, Akiyama T, Muto T, Yajima S, Sue M, Seo M, Kanno Y, Kamo T, Endo A, Nambara E, Hirai N, Ohnishi T, Cutler SR, Todoroki Y. Nat Chem Biol 10 477-482 (2014)
  4. Mosquna A, Peterson FC, Park SY, Lozano-Juste J, Volkman BF, Cutler SR. Proc Natl Acad Sci U S A 108 20838-20843 (2011)
  5. Li W, Wang L, Sheng X, Yan C, Zhou R, Hang J, Yin P, Yan N. Cell Res 23 1369-1379 (2013)
  6. Yuan X, Yin P, Hao Q, Yan C, Wang J, Yan N. J Biol Chem 285 28953-28958 (2010)
  7. Kepka M, Benson CL, Gonugunta VK, Nelson KM, Christmann A, Grill E, Abrams SR. Plant Physiol 157 2108-2119 (2011)
  8. Puli MR, Raghavendra AS. J Exp Bot 63 1349-1356 (2012)
  9. Ye Y, Zhou L, Liu X, Liu H, Li D, Cao M, Chen H, Xu L, Zhu JK, Zhao Y. Plant Physiol 173 2356-2369 (2017)
  10. Soon FF, Suino-Powell KM, Li J, Yong EL, Xu HE, Melcher K. PLoS One 7 e47857 (2012)
  11. Zhang Q, Kong X, Yu Q, Ding Y, Li X, Yang Y. Plant Signal Behav 14 1670596 (2019)
  12. Van Overtveldt M, Heugebaert TS, Verstraeten I, Geelen D, Stevens CV. Org Biomol Chem 13 5260-5264 (2015)
  13. Chen Y, Zhang JB, Wei N, Liu ZH, Li Y, Zheng Y, Li XB. Plant Mol Biol 107 499-517 (2021)
  14. Zhang X, Wu W, Chen Z. Acta Crystallogr Sect F Struct Biol Cryst Commun 68 479-482 (2012)
  15. Lee Y, Heo S, Lee S. Methods Mol Biol 2462 1-16 (2022)


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