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A ternary complex of Hydroxycinnamoyl-CoA Hydratase-Lyase (HCHL) with acetyl-Coenzyme A and vanillin gives insights into substrate specificity and mechanism.

Source organism: Pseudomonas fluorescens
Biochemical function: feruloyl-CoA hydratase/lyase activity
Biological process: isoprenoid catabolic process
Cellular component: not assigned

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EC 4.1.2.61: Feruloyl-CoA hydratase/lyase

Reaction catalysed:
(1a) feruloyl-CoA + H(2)O = 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoyl-CoA
Systematic name:
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoyl-CoA vanillin-lyase (acetyl-CoA-forming)
Alternative Name(s):
  • 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA:vanillin lyase (acetyl-CoA-forming)

GO terms

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Sequence family

Pfam Protein family (Pfam)
PF00378
Domain description: Enoyl-CoA hydratase/isomerase
Occurring in:
  1. Hydroxycinnamoyl-CoA hydratase-lyase
  2. Hydroxycinnamoyl-CoA hydratase-lyase
  3. Hydroxycinnamoyl-CoA hydratase-lyase
  4. Hydroxycinnamoyl-CoA hydratase-lyase
The deposited structure of PDB entry 2vsu contains 3 copies of Pfam domain PF00378 (Enoyl-CoA hydratase/isomerase) in Hydroxycinnamoyl-CoA hydratase-lyase. Showing 1 copy in chain B.
The deposited structure of PDB entry 2vsu contains 1 copy of Pfam domain PF00378 (Enoyl-CoA hydratase/isomerase) in Hydroxycinnamoyl-CoA hydratase-lyase. Showing 1 copy in chain C.
The deposited structure of PDB entry 2vsu contains 1 copy of Pfam domain PF00378 (Enoyl-CoA hydratase/isomerase) in Hydroxycinnamoyl-CoA hydratase-lyase. Showing 1 copy in chain E.
The deposited structure of PDB entry 2vsu contains 1 copy of Pfam domain PF00378 (Enoyl-CoA hydratase/isomerase) in Hydroxycinnamoyl-CoA hydratase-lyase. Showing 1 copy in chain F.

InterPro InterPro annotations
IPR018376
Domain description: Enoyl-CoA hydratase/isomerase, conserved site
Occurring in:
  1. Hydroxycinnamoyl-CoA hydratase-lyase
  2. Hydroxycinnamoyl-CoA hydratase-lyase
  3. Hydroxycinnamoyl-CoA hydratase-lyase
  4. Hydroxycinnamoyl-CoA hydratase-lyase
IPR001753
Domain description: Enoyl-CoA hydratase/isomerase
Occurring in:
  1. Hydroxycinnamoyl-CoA hydratase-lyase
  2. Hydroxycinnamoyl-CoA hydratase-lyase
  3. Hydroxycinnamoyl-CoA hydratase-lyase
  4. Hydroxycinnamoyl-CoA hydratase-lyase
IPR029045
Domain description: ClpP/crotonase-like domain superfamily
Occurring in:
  1. Hydroxycinnamoyl-CoA hydratase-lyase
  2. Hydroxycinnamoyl-CoA hydratase-lyase
  3. Hydroxycinnamoyl-CoA hydratase-lyase
  4. Hydroxycinnamoyl-CoA hydratase-lyase

Structure domains

CATH CATH domains
6.10.250.2850
Class: Special
Architecture: Helix non-globular
Topology: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homology: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Occurring in:
  1. Hydroxycinnamoyl-CoA hydratase-lyase
  2. Hydroxycinnamoyl-CoA hydratase-lyase
  3. Hydroxycinnamoyl-CoA hydratase-lyase
  4. Hydroxycinnamoyl-CoA hydratase-lyase
The deposited structure of PDB entry 2vsu contains 3 copies of CATH domain 6.10.250.2850 (Single alpha-helices involved in coiled-coils or other helix-helix interfaces) in Hydroxycinnamoyl-CoA hydratase-lyase. Showing 1 copy in chain B.
The deposited structure of PDB entry 2vsu contains 1 copy of CATH domain 6.10.250.2850 (Single alpha-helices involved in coiled-coils or other helix-helix interfaces) in Hydroxycinnamoyl-CoA hydratase-lyase. Showing 1 copy in chain C.
The deposited structure of PDB entry 2vsu contains 1 copy of CATH domain 6.10.250.2850 (Single alpha-helices involved in coiled-coils or other helix-helix interfaces) in Hydroxycinnamoyl-CoA hydratase-lyase. Showing 1 copy in chain E.
The deposited structure of PDB entry 2vsu contains 1 copy of CATH domain 6.10.250.2850 (Single alpha-helices involved in coiled-coils or other helix-helix interfaces) in Hydroxycinnamoyl-CoA hydratase-lyase. Showing 1 copy in chain F.
3.90.226.10
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: 2-enoyl-CoA Hydratase; Chain A, domain 1
Homology: 2-enoyl-CoA Hydratase; Chain A, domain 1
Occurring in:
  1. Hydroxycinnamoyl-CoA hydratase-lyase
  2. Hydroxycinnamoyl-CoA hydratase-lyase
  3. Hydroxycinnamoyl-CoA hydratase-lyase
  4. Hydroxycinnamoyl-CoA hydratase-lyase
The deposited structure of PDB entry 2vsu contains 3 copies of CATH domain 3.90.226.10 (2-enoyl-CoA Hydratase; Chain A, domain 1) in Hydroxycinnamoyl-CoA hydratase-lyase. Showing 1 copy in chain B.
The deposited structure of PDB entry 2vsu contains 1 copy of CATH domain 3.90.226.10 (2-enoyl-CoA Hydratase; Chain A, domain 1) in Hydroxycinnamoyl-CoA hydratase-lyase. Showing 1 copy in chain C.
The deposited structure of PDB entry 2vsu contains 1 copy of CATH domain 3.90.226.10 (2-enoyl-CoA Hydratase; Chain A, domain 1) in Hydroxycinnamoyl-CoA hydratase-lyase. Showing 1 copy in chain E.
The deposited structure of PDB entry 2vsu contains 1 copy of CATH domain 3.90.226.10 (2-enoyl-CoA Hydratase; Chain A, domain 1) in Hydroxycinnamoyl-CoA hydratase-lyase. Showing 1 copy in chain F.

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