› 2zsh

X-ray diffraction
1.8Å resolution

Structural basis of gibberellin(GA3)-induced DELLA recognition by the gibberellin receptor

Released:
DOI:
PDB entry 2zsh coloured by chain, front view.
PDB entry 2zsh coloured by chain, side view.
PDB entry 2zsh coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Gibberellin-induced DELLA recognition by the gibberellin receptor GID1.
Murase K, Hirano Y, Sun TP, Hakoshima T
Nature 456 459-63 (2008)
PMID: 19037309

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192302 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gibberellin receptor GID1A Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 39.28 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9MAA7 (Residues: 1-344; Coverage: 100%)
Gene names: At3g05120, CXE10, GID1A, GID1L1, T12H1.8
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase
DELLA protein GAI Chain: B
Molecule details ›
Chain: B
Length: 110 amino acids
Theoretical weight: 12.28 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LQT8 (Residues: 11-113; Coverage: 19%)
Gene names: At1g14920, F10B6.34, GAI, RGA2
Sequence domains: Transcriptional regulator DELLA protein N terminal
Structure domains: Transcriptional regulator DELLA, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand GA3 1 x GA3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P43212
Unit cell:
a: 82.018Å b: 82.018Å c: 130.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.229
Expression system: Escherichia coli

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Citations

92 review citations

Vierstra RD. (2009)
91 more

9 mentions without citation

Liu et al. (2019)
8 more